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 廣州健侖生物科技?有限公司 

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【公司名稱】 廣州健侖生物科技有限公司
【】    楊永漢 

【】
【騰訊 】
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-3室

【企業(yè)文化宣傳】

八。伏室溫勻漿管中2-3深所鐘。
九。每二十毫升lv仿添RNA處劑四毫升。安帽取樣，用力搖十五秒。冰上傴伏十深所鐘。
十。于12000°心殿深所鐘離十五，火矢分為下者lv仿相、相間與水相四時(shí)。RNA全存在水相。
十一。轉(zhuǎn)不過(guò)一新管水80%加無(wú)水乙醇1 /二卷（腮96-100%，室溫）。大分為三十秒之當(dāng)。
意：所有之心也應(yīng)在室溫下行。
十二。將一樣裝在一個(gè)五十毫升之收管HiBind®RNA馬克西欄（供。。之™HiBind RNA馬克西列之大量為二十五毫升（大者積可載前后。）于二千x深所鐘離三木。棄過(guò)仍至法二十二。
十三。滌塔Wash Buffer吾以移液十毫升直入RNA大旋柱。在五十x g離三深所鐘，棄50ml收管。此kangyi棄五十毫升收管，換一新之50ml無(wú)RNase之離管（供）免RNase污于下一步驟前。
四。以移液十毫升洗液稀釋RNA II以乙醇滌塔。離心機(jī)與出流。在道中用總管十五。
8。孵化室溫勻漿理中2-3分鐘。
9。添加每二十毫升lv仿RNA處理劑4毫升。頭盔取樣，大力岌15秒鐘。雪上哺育十分鐘。
10。喺12000°C.離心三個(gè)字，混合物分為低酚lv仿相、相間同上水相4個(gè)階段。RNA*保持喺水相中。
11。Transfer no more than 80% of the aqueous phase to a fresh tube and add 1/2 volume of absolute ethanol（~96-100%，room temperature）.zui大嘅速度為30秒嘅旋渦。
注意：所有嘅離心步驟應(yīng)喺室溫下進(jìn)行。
12。將成樣品組裝喺一個(gè)五十毫升嘅有冇收集理HiBind®RNA馬克西欄（講嚇嘢喇?。?。嘅™HiBind RNA馬克西部嘅zui大蓋為25毫升（較大嘅體積可以載入先后。）于2000 x g離心3分鐘。擗流過(guò)并繼續(xù)去步驟12。
13。洗滌塔Wash Buffer我移液15毫升直接進(jìn)入RNAzui大旋轉(zhuǎn)柱。喺5000 x  g離心3分鐘，棄50ml有冇收集理。呢個(gè)系強(qiáng)烈建議放棄五十毫升有冇收集理，換個(gè)新嘅50ml無(wú)RNase嘅離心理（講嚇嘢喇?。┨舆^(guò)RNase污染到落個(gè)步驟之前。
14。移液15毫升清洗液稀釋RNA II用乙醇洗滌塔。離心機(jī)同排出流。喺步驟15中重用集合理。

Eight. 2-3 deep clocks in a room temperature homogenate.
Nine. Twenty milliliters of LV are four milliliters for adding RNA. Sample the helmet and shake for fifteen seconds. The ten volt deep ice slouch clock.
Ten. In the 12000 degree heart Temple deep Zhongli fifteen, were divided into fire bolt LV imitation phase, interphase and phase four. All RNA exists in the water phase.
Eleven. A new tube of water 80% plus 1 / two without water (96-100%, room temperature). It is divided into thirty seconds.
Meaning: all hearts should also go down at room temperature.
Twelve. The same is placed in a fifty ml collection tube HiBind RNA Maxi bar (for.. It is the HiBind RNA listed a large number of Maxi twenty-five ml (large volume can be loaded before and after.) In two thousand x deep by Miki zhongli. Discarded to law twenty-two.
Thirteen. Wash Buffer I take the washing tower pipette ten ml into the RNA spin column. Fifty x g from three deep clocks, discarded 50ml receiving tube. This protest abandons fifty milliliters to receive the tube and changes a new 50ml free RNase off tube (supply) to avoid RNase pollution before the next step.
Four. Dilute RNA II with a ten milliliter lotion for alcohol polyester pagoda.  Centrifuge and flow. The main pipe is fifteen in the road.
8. Incubate room temperature for 2-3 minutes.
9. Add 4 ml of twenty milliliter LV per milliliter for RNA treatment. The helmet sampling, Ji vigorously for 15 seconds. The snow is fed for ten minutes.
10. In 12000 ~ C. centrifugal three words, divided into low LV phenol mixture, and aqueous phase in imitation of 4 stages. RNA*保持喺水相中。
11. Transfer no more than the aqueous phase to 80% of a fresh tube and add 1/2 volume of absolute ethanol (~96-100%, room, temperature). The maximum speed of 30 seconds, vortex.
Note: all my steps should be carried out at room temperature in centrifugal.
12. The sample assembly in a fifty ml, have collected HiBind RNA Maxi column (about the scare yeah!). It's HiBind RNA Mark's biggest western 25 ml cover (larger volume, you can load it.) 2000 x g centrifugation for 3 minutes. To go through and continue to step 12.
13. Washing tower Wash Buffer I move 15 ml directly into the RNA maximum rotary column. In 5000 x g centrifuge for 3 minutes, 50ml have collected and abandoned. It is strongly recommended to give up fifty ml has been collected, a new 50ml's free RNase. From the psychological (speak ye scared!) Escape from RNase pollution until the drop step.
14. The 15 milliliter cleaning solution is diluted to RNA II with ethanol washing tower. The centrifuge is the same as the discharge flow. In step 15 set reasonable reuse.