軍團(tuán)菌診斷血清（凝集法）

廣州健侖生物科技有限公司

廣州健侖長(zhǎng)期供應(yīng)：軍團(tuán)菌、諾如病毒、流感病毒等傳染病系列的快速檢測(cè)試劑盒。

軍團(tuán)菌的檢測(cè)試劑盒包括：軍團(tuán)菌尿液抗原檢測(cè)試劑盒、軍團(tuán)菌抗體快速檢測(cè)卡（膠體金法）、軍團(tuán)菌抗原快速檢測(cè)卡（膠體金法）、軍團(tuán)菌水樣檢測(cè)試劑盒、軍團(tuán)菌乳膠凝集試劑盒（軍團(tuán)菌診斷血清）、嗜肺軍團(tuán)菌核酸熒光PCR檢測(cè)試劑盒。

我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒：包括傳染病系列、免疫組化系列、診斷血清等產(chǎn)品。

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【日本生研軍團(tuán)菌診斷血清（凝集法）】

200549 軍團(tuán)菌診斷血清  套裝 2ml  10支

214997        　pneumophila１群    嗜肺軍團(tuán)菌 2ML

215000        　pneumophila２群 規(guī)格：2ML

215017        　pneumophila３群 規(guī)格：2ML

215024        　pneumophila４群 規(guī)格：2ML

215031        　pneumophila５群 規(guī)格：2ML

215048        　pneumophila６群 規(guī)格：2ML

215055          bozemanii   博茲曼軍團(tuán)菌 2ML

215062          dumoffii    杜莫夫軍團(tuán)菌    2ML

215079          gormanii    戈?duì)柭妶F(tuán)菌 2ML

215086          micdadei    米克戴德軍團(tuán)菌 2ML

215727        　pneumophila7群 規(guī)格：2ml

215734        　pneumophila8群 規(guī)格：2ml

293572        　pneumophila9群 規(guī)格：2ml

293589        　pneumophila10群 規(guī)格：2ml

293626        　pneumophila11群 規(guī)格：2ml

293633        　pneumophila12群 規(guī)格：2ml

293640        　pneumophila13群 規(guī)格：2ml

293657        　pneumophila14群 規(guī)格：2ml

293664        　pneumophila15群 規(guī)格：2ml

【日本生研】

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【公司名稱】 廣州健侖生物科技有限公司

【市 場(chǎng) 部】    楊永漢

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【騰訊Q Q】 2042552662

【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

1. 細(xì)菌培養(yǎng)法
軍團(tuán)菌為革蘭氏陰性桿菌，專性需氧，胞內(nèi)寄生菌。軍團(tuán)菌zui初是從以軍團(tuán)菌病人肺組織感染的豚鼠中分離出來的。這種方法雖然有較高的可靠性，但非常昂貴，而且費(fèi)時(shí)費(fèi)力，不久就被平板培養(yǎng)法所取代。目前標(biāo)準(zhǔn)培養(yǎng)基為活性碳酵母浸膏瓊脂平板，也稱軍團(tuán)菌生長(zhǎng)平板(BCYE)。接種在 BCYE 平板上的樣品在溫度為35-37ºC，培養(yǎng)10天，在濃度為2.5% CO2 的環(huán)境下培養(yǎng)更有利于軍團(tuán)菌的生長(zhǎng)。軍團(tuán)菌的菌落通常呈白色、灰色、有熒光。軍團(tuán)菌在 BCYE 平板上生長(zhǎng)而在平板和半光氨酸缺失 BCYE-Cys 平板上不生長(zhǎng)。
2.血清學(xué)玻片凝集法
軍團(tuán)菌感染l周左右可檢測(cè)出血清中軍團(tuán)菌特異性IgM抗體，2周左右可檢測(cè)到特異性IgG抗體。
現(xiàn)已發(fā)現(xiàn)嗜肺軍團(tuán)菌有15個(gè)血清型。其中，嗜肺軍團(tuán)菌血清1型（Lp1）與人類疾病關(guān)系z(mì)ui密切，其次為血清4型（Lp4）和6型（Lp6）。
3.核酸擴(kuò)增法
核酸擴(kuò)增技術(shù)，即聚合酶鏈?zhǔn)椒磻?yīng)（polymerase chainreaction，PCR），其基本原理是設(shè)計(jì)、合成兩條寡核苷酸，作為引物，對(duì)應(yīng)于待測(cè)病原微生物某一段特異性序列的兩端，然后在體外模擬DNA體內(nèi)復(fù)制的過程反復(fù)擴(kuò)增，使靶序列放大上萬倍甚至上百萬倍而被檢測(cè)出來。
常規(guī)PCR：鑒定標(biāo)準(zhǔn)是通過電泳來判斷是否有擴(kuò)增的核酸片段以及擴(kuò)增產(chǎn)物的大小是否正確。
實(shí)時(shí)熒光PCR：通過對(duì)實(shí)時(shí)熒光PCR反應(yīng)的每一個(gè)循環(huán)產(chǎn)物進(jìn)行熒光信號(hào)的實(shí)時(shí)監(jiān)測(cè)來判斷分析。

Bacterial culture
Legionella is Gram-negative bacilli, obligate aerobic, intracellular parasites. Legionella was first isolated from guinea pigs infected with lung tissue of Legionnaires' patients. Although this method has high reliability, but very expensive, but also time-consuming and laborious, and soon was replaced by plate culture method. The current standard medium is activated carbon yeast extract agar plate, also known as Legionella growth plate (BCYE). The samples inoculated on BCYE plates grew at a temperature of 35-37ºC for 10 days and cultured at a concentration of 2.5% CO 2 more conducive to Legionella growth. Legionella colonies are usually white, gray, fluorescent. Legionella grew on BCYE plates and did not grow on plates and half-my-leucine-deficient BCYE-Cys plates.
2. Serological slide agglutination method
Legionella infection can be detected about 1 week in the serum of Legionella specific IgM antibodies, about 2 weeks to detect specific IgG antibodies.
Legionella pneumophila has been found in 15 serotypes. Among them, Legionella pneumophila serotype 1 (Lp1) has the closest relationship with human diseases, followed by Lp4 and Lp6.
3 nucleic acid amplification method
The principle of nucleic acid amplification, that is, polymerase chain reaction (PCR), is to design and synthesize two oligonucleotides, which serve as primers and correspond to the two ends of a specific sequence of the pathogenic microorganism to be tested. Then in vitro replication of DNA replication in vivo repeated amplification process, the target sequence to enlarge tens of thousands or even millions of times were detected.
Conventional PCR: The standard of identification is determined by electrophoresis to determine whether the amplified nucleic acid fragment and the size of the amplified product are correct.
Real-time fluorescence PCR: Real-time fluorescence PCR reaction through real-time monitoring of each signal to determine the fluorescence signal analysis.