核酸試劑 諾如病毒G1/G2檢測試劑盒(PCR-熒光探針法)

廣州健侖生物科技有限公司

準(zhǔn)備使用lyo master混合物（各8孔條），用于檢測諾如病毒G1和諾如病毒G2包括內(nèi)部對照。
Ready to use lyo master mix (8-well strips) for detection of norovirus G1 and norovirus G2 including an internal control

核酸試劑 諾如病毒G1/G2檢測試劑盒(PCR-熒光探針法)

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核酸試劑 諾如病毒G1/G2檢測試劑盒(PCR-熒光探針法)

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

方法如下： 
1．人IgG聚合物的制備 在5ml含40mg/ml人IgG的0.1mol/L pH7.0磷酸緩沖溶液中，加入2.5%戊二醛溶液1ml，邊加邊攪，5min即出現(xiàn)混濁，逐現(xiàn)大塊膠塊，放置30min后，用研缽將凝膠磨細(xì)，繼用1.0mol/L pH7.0磷酸緩沖溶液反復(fù)洗滌3次，末次加蒸餾水至20ml，即為人IgG聚合物懸液。 
2．免疫吸收法
將待吸收的抗SIgG血清加入待量IgG聚合物懸液，置室溫攪拌60min,離心沉淀，上清液即稀釋1倍的純化抗SIgA血清。如用IgG聚合物作少量分次吸收，其效果更好。 
（八）伊文氏藍(lán)（Evans blue）襯染法 
用0.01%伊文氏藍(lán)的0.01mol/L pH7.2PBS稀釋熒光抗體，可將背景細(xì)胞和組織染色，呈紅色熒光，與特異性黃綠色熒光形成鮮明的對比，減少了非特異性熒光，宜作常規(guī)應(yīng)用。伊文氏藍(lán)一般先配成1%溶液，保存于4℃，用前再稀釋至0.01%用以和然釋熒光抗體。 此外，還可以用胰酶消化組織切片或用10%牛血清蛋白封閉法等消除非特異性染色，提高特異性染色。 

Methods as below: 
1. Preparation of human IgG polymer In 5ml 0.1mol / L pH7.0 phosphate buffer solution containing 40mg / ml human IgG, add 1ml of 2.5% glutaraldehyde solution, while adding stirring, turbidity appeared 5min, After placing for 30min, the gel is ground in a mortar, washed repeatedly with 1.0mol / L phosphate buffer solution pH7.0 three times, distilled water last added to 20ml, which is human IgG polymer suspension.
2. Immunostimulation
The anti-SIgG serum to be absorbed was added to the suspension of IgG polymer to be measured, stirred for 60 minutes at room temperature, and centrifuged. The supernatant was diluted 1 time with purified anti-SIgA serum. Such as using IgG polymer as a small amount of fractional absorption, the effect is better.
(Eight) Evans blue lining method
Fluorescent antibodies were diluted with 0.01 mol / L pH 7.2 PBS at 0.01% Evans blue to stain background cells and tissues, showing red fluorescence in stark contrast to specific yellow-green fluorescence and reducing nonspecific fluorescence. Conventional application. Evans blue generally first dubbed 1% solution, stored at 4 ℃, before use and then diluted to 0.01% for natural release of fluorescent antibodies. In addition, you can try to digest tissue sections with trypsin or 10% bovine serum albumin and other non-specific staining to eliminate and improve the specific staining.